目的 建立同时测定藏药诃子中没食子酸、诃子次酸、柯里拉京、没食子酸乙酯、诃子鞣酸、鞣花酸和五没食子酰葡萄糖的HPLC方法,测定不同产地诃子药材中7种鞣质类有效成分的含量。方法 采用色谱柱为Hypersil ODS2 C18(4.6 mm×250 mm,5 μm);流动相为乙腈(A)-0.05%甲酸水(B),梯度洗脱,紫外检测波长为290 nm;流速1 mL·min-1。结果 7种有效成分线性关系良好(r＞0.999 8),加样回收率为95.2%~98.4%;8个产地不同品种的诃子中均含该7个被测成分,但含量差异大,其中诃子品种中海南、广西、广东和新疆几个产地的7种鞣质类有效成分的总含量较高;绒毛诃子品种中广东和广西两个产地的7种鞣质类有效成分的总含量较高。结论 该方法快速简便,分离效果好,重复性好,可为诃子的质量控制提供重要参考。
Abstract
OBJECTIVE To develop an HPLC method for simultaneous determination of seven tannins in Chebulae Fructus, including gallic acid, chebulic acid, corilagin, ethyl gallate, ellagic acid, chebulagic acid and 1,2,3,4,6-O-pentagalloylglucose and determine the contents of the seven tannins in Chebulae Fructus Retz from different areas.METHODS The HPLC analysis was carried out on an Hypersil ODS2 C18 (4.6 mm×250 mm,5 μm) column with acetonitrile (A) and 0.05% formic acid solution in water (B) as mobile phase in a linear gradient elution mode. The UV detection wavelength was set at 290 nm and the flow rate was 1.0 mL·min-1.RESULTS The calibration curves of the seven tannins all showed good linearity (r＞0.999 8). The recovery rates were in the range of 95.2% to 98.4%. All the seven tannins could be detected in the two kinds of Chebulae Fructus Retz from eight regions, but the amounts of these tannins varied significantly. The contents of the seven tannins active ingredients in Chebulae Fructus of Terminalia chebula Retz from Hainan, Guangxi, Guangdong and Xinjiang were much higher than those from other areas, while those in Chebulae Fructus of Terminalia chebula Retz. var. tomentella Kurt were higher in Guangdong and Guangxi than other areas.CONCLUSION The method is proved to be accurate and valid, and can be used for the quality control of Terminalia chebula Retz.
关键词
高效液相色谱法 /
藏药诃子 /
鞣质 /
诃子次酸 /
五没食子酰葡萄糖
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Key words
HPLC /
Chebulae Fructus /
tannin /
chebulagic acid /
1,2,3,4,6-O-pentagalloyl glucose
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中图分类号：
R284.1
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参考文献
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基金
江西民族传统药现代科技与产业发展协同创新中心2014年度协同创新项目资助(JXXT201403019);江西省自然基金科学项目资助(20161BAB205222)
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